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The Chemical PE Thread

Originally Posted by onemanorgy
Iwant to get a centrifuge and do my own priaprism shots

Was actually wondering why nobody mentions this here or on other forums.

I don’t know the whole process of drawing/injecting, but it doesn’t seem that complex in the youtube vids (but then again, it’s youtube).

They seem to go for like $200-300 on ebay. Not sure of rpm’s needed.
There could be a patent doc related to priapus shots out there somewhere to get more of an overview on them.

With some luck, you can get a used lab centrifuge for around 50 USD. It is important that the speed can be regulated.
It doesn’t have to be an expensive PRP extraction centrifuge.

Technically and in theory, the extraction process doesn’t seem that difficult. There are however some variations on the preparation process (see http://orthodoc.aaos.org/WilliamFBe…Variability.pdf), especially the parameters speed and time of the centrifuge. See also http://www.academicjournals.org/art…,%20et%20al.pdf

In the following description a centrifuge speed of 2400 rpm is recommended, whereas I have read in other places that the speed should rather be at 1000 rpm granting a force equaling 150-200g (note: physically not correct). Higher speeds may cause the platelets to seep in the blood and thus create platelet poor plasma.

Quote
Manual Protocol
CURASAN METHOD (Germany): 10 Most commonly used method. The blood sample is drawn into a citrated tube. The sample tube is then spun in a standard centrifuge for 10 minutes at 2400 rpm to produce PPP. The PPP and buffy coat is taken up into a syringe with a long cannula and an additional air-intake cannula. A second centrifugation(15 minutes at 3600 rpm) is performed to concentrate the platelets. The second supernatant is also taken up by along cannula and an air-intake cannula. For each 8 mL of blood, the volume of supernatant is about 0.6-0.7 mL; this is the PRP, to be used for then surgical procedure. Just before the time of the application, the PRP is combined with an equal volume of a sterile saline solution containing 10% calcium chloride (a citrate inhibitor that allows the plasma to coagulate) and 100 you/mL of sterile bovine thrombin (an activator that allows polymerization of the fibrin into an insoluble gel, which causes the platelets to degranulate and release theindicated mediators and cytokines); the result should be asticky gel that will be relatively easy to apply to the surgicaldefects. The PPP can be stored for use as a protective barrier over the wound.


After centrifuging process, the platelets together with leucocytes are then in the buffy layer (C) http://www.ijds.in/article-image-13…LASMA_PRP .jpg
The ratio of PRP to total withdrawn blood should be in the range of 1:8 to 1:10, or depending upon concentration levels even a bit lower.

The technicalities seem feasible. The biggest problem I see is withdrawing ones own blood.

More info about platelets:
http://www.forhair.com/autologous-p…ich-plasma-prp/
http://www.dermavita.bg/english/ind…id=19&Itemid=19


Sssnrgd..

.Clickdiclack.Rrndhgzzirp..

."Wow!"*

EDIT: In the picture, the buffy layer is (B).


Sssnrgd..

.Clickdiclack.Rrndhgzzirp..

."Wow!"*

Look guys with all the fake riptropin shit and fake HGH out there. I’d rather you inject your penis with your own blood than egg albumin. IWll buy a centrifuge next month and some needles and some calcium and d waterver he puts in his cocktail might add some HGH in it to. So if you guys wanted to help assist me in being the guina pig I’m down.

Also other than Dr runels writing it in a book I’ve never seen yet. I’m convinced nobody has injected HGH in ther epenis other than me. Theres absolutely no way for it to stop going systematic as it still works in your system 5 hours you injected it. I say this becuase 2nd time I injected didnt want to say nothing on here, I felt it hit my blood stream and I got the worst headache on my nordic bone and top of my head in my spine and somewhat in my legs and a little in my wrist. It felt like that hollow pain you get punching a punching bag all day the typ eo pain that makes your bones grow and become thick. Legitly the myths you hear on the internet of ‘ohh this one guy injected 4 iu of HGH in his dick” thats a bunch of bullshit. They either got fkae shit or there lying, growth hormone is the master hormone of the entire body. Nothing to play with there is no possible way they injected 4 iu in the penis as I only injecte 1 iu and got that side effect, then I knew to dose it half an iu in my right CC as that caused the stystemtic effect and .75 iu in my left CC. After every HGH shot to you get like your erection quality is fucked for about a week later till your dht in your balls kicks back in.

How would you want to withdraw your own blood without assistance from a nurse?


Sssnrgd..

.Clickdiclack.Rrndhgzzirp..

."Wow!"*

Originally Posted by onemanorgy
Look guys with all the fake riptropin shit and fake HGH out there. I’d rather you inject your penis with your own blood than egg albumin. IWll buy a centrifuge next month and some needles and some calcium and d waterver he puts in his cocktail might add some HGH in it to. So if you guys wanted to help assist me in being the guina pig I’m down.

Also other than Dr runels writing it in a book I’ve never seen yet. I’m convinced nobody has injected HGH in ther epenis other than me. Theres absolutely no way for it to stop going systematic as it still works in your system 5 hours you injected it. I say this becuase 2nd time I injected didnt want to say nothing on here, I felt it hit my blood stream and I got the worst headache on my nordic bone and top of my head in my spine and somewhat in my legs and a little in my wrist. It felt like that hollow pain you get punching a punching bag all day the typ eo pain that makes your bones grow and become thick. Legitly the myths you hear on the internet of ‘ohh this one guy injected 4 iu of HGH in his dick” thats a bunch of bullshit. They either got fkae shit or there lying, growth hormone is the master hormone of the entire body. Nothing to play with there is no possible way they injected 4 iu in the penis as I only injecte 1 iu and got that side effect, then I knew to dose it half an iu in my right CC as that caused the stystemtic effect and .75 iu in my left CC. After every HGH shot to you get like your erection quality is fucked for about a week later till your dht in your balls kicks back in.

hey oneman.

I have some pharmacy bought hgh in the fridge . I plan on injecting 2 ui /3 times a week. Do you inject hgh IC or SubQ(penis) ?

That;s weird GH having an effect of lowering EQ. It’s even used to increase penile eNOS.
http://www.sciencedirect.com/scienc…022534701624406
GROWTH HORMONE ENHANCES REGENERATION OF NITRIC OXIDE SYNTHASE-CONTAINING PENILE NERVES AFTER CAVERNOUS NERVE NEUROTOMY IN RATS
“We believe that GH administration may present a new and more physiologic approach to the treatment of erectile dysfunction after radical pelvic surgery.”

Where have bought your GH ?


Starting stats: 6.4" / 5.6" Current Stats: 7.4" / 5.8" Short term goal: 7" / 6" Long term goal: 8" / 6.5"

Hey Guys Im back after a long sojourn…

I have injected HGH directly also, and by the advice of Dr Runels, whom I got a Priapus Shot from as well, 2 of them. I have also managed to successfully perform my own PRP injection successfully via the use of my centrifuge and a PRP kit. I just used the kit for my wifes boobs, and it worked well and made her nipples fuller as if they exfoliated out to their potential and became full bottle tip nipples when they were more flat. I could have used it on my penis for a Priapus Shot but I didnt. I had to draw blood all that, but my kit included it all. It was a great kit out of South Africa. So the reality is you can do it yourself and it has been done and usefully so I might add, my wifes boobs also gained in general volume.

I have read somewhere that there exists a synergy between IGF-1 and HGH also and have noted myself that when I take HGH in my penis or IGF-1 or together that the PGE-1 is more effective to the point of worry sometimes. But I presume this is due to the level of Testosterone level raising due to the HGH or IGF-1. I have also noted via other means that raise my testosterone lick HCG or direct T or even DHT, all seem to make the PGE-1 work better, stronger or longer.

I got 10 PRP kits, but limited on the blood draw stuff, dont need calcium chloride because I have a mechanical activator now. PRP kits are costly so do your best to find any you can afford. The ones I got from South Africa cost 50 bucks at first now they are a 100 but they no longer export to the USA so that sucks… Anyway I found someone else but it like is a one time deal. I just moved and my lab is not setup so some time down the line I will get to reporting on my own Priapus Shot experiments

Inuic

Originally Posted by Doubleweener
How would you want to withdraw your own blood without assistance from a nurse?

If you’re brave enough.

I’ve managed to insert an IV line on myself. Drawing blood might take too long and hurt a lot.

Interesting thread.


"Men are gifted with two heads, sadly they do not

have enough blood to run

both at the same time" by anonymous

Anyone done any research on topical Glutathione?

Glutathione through glans penis

This might be something if it can impact collagen linking and unlinking…

Inuic

TB4 Goes well with PGE-1 to reduce inflammation

TB4 including its role as an actin sequestrant and its roles in inflammation and immune function.

Thymosin Beta-4, or TB4, sometimes called TB500, is a 44-amino-acid peptide and signaling protein with divergent functions throughout the body. TB4 is found in nearly all types of living tissue. Initially discovered in its role as an actin-sequestration agent, TB4 is now understood in humans and animal models to play a role in wound-healing, neurological regeneration, cardiac regeneration, immune function, cell migration, and blood vessel formation.

Thymosins interact with actin and regulate its growth and function and thereby the growth and function of both smooth and skeletal muscle-tissue; thymosin beta-4 binds with G-actin (a monomeric or single-strand form of actin) and stabilizes it; along with ATP and another monomeric filament, this complex forms F-actin, the polymeric thin filament found in muscle tissue. It is in this way that TB4 plays a key role in tissue regeneration, vascularization, and wound repair.

The ways thymosin beta-4 interacts with actin in different types of tissue is not limited to pure regulation of growth in contractile and smooth tissue:

After injury, thymosin B(4), is released by platelets, macrophages and many other cell types to protect cells and tissues from further damage and reduce apoptosis, inflammation and microbial growth. Thymosin B(4) binds to actin and promotes cell migration, including the mobilization, migration, and differentiation of stem/progenitor cells, which form new blood vessels and regenerate the tissue. Thymosin B(4) also decreases the number of myofibroblasts in wounds, resulting in decreased scar formation and fibrosis.[1]

TB4 has several moonlighting roles, such as modulation of inflammation in different types of tissue, and indirect (as opposed to direct via chemotaxis) influence on immune function.

Thymosin beta 4 is largely unfolded or perhaps completely unfolded in solution. Based on the paradigm introduced by Wright and Dyson that unfolded proteins may have multiple functions based on their ability to recognize numerous ligands, the flexible structure of thymosin beta 4 may facilitate the recognition of a variety of molecular targets, thus explaining the plethora of functions attributed to thymosin beta 4. Furthermore, if multiple ligands bind to thymosin beta 4, then it is possible that thymosin beta 4 has a unique integrative function that links the actin cytoskeleton to important immune and cell growth-signaling cascades. [3,4]

Thymosin beta-4 is a potent wound healing accelerator, and many of the current (experimental) clinical uses focus on its ability to speed healing in disease states. In addition to its promising effects in inflammatory and immune disorders, TB4 has been shown to be beneficial and important in targeted tissue regeneration in disease states such as advanced diabetes mellitus as stated by Treadwell

naturally occurring, endogenous repair molecule, thymosin beta 4 (TB4), has many biological activities that promote dermal repair. It is released by platelets at the site of injury and initiates the repair cascade. TB4 accelerated dermal healing

TB4 likely acts to repair and regenerate wounds by promoting cell migration and stem cell mobilization and differentiation, and by inhibiting inflammation, apoptosis, and infection. We conclude that TB4 is a multifunctional regenerative peptide important in dermal repair.[5]

The ability of exogenous TB4 to reduce fibrotic tissue formation, reduce damage from ischemia/reperfusion, and accelerate wound-healing …

[1]Goldstein AL, Hannappel E, Sosne G, Kleinman HK. Thymosin ?4: a multi-functional regenerative peptide. Basic properties and clinical applications. Expert Opin Biol Ther. 2012 Jan;12(1):37-51.

[3]Wright PE, Dyson HJ. .Intrinsically unstructured proteins: re-assessing the protein structure-function paradigm. J Mol Biol. 1999 Oct 22;293(2):321-31

[4]Bubb MR. Thymosin beta 4 interactions. Vitam Horm. 2003;66:297-316.

[5]Treadwell T, Kleinman HK, Crockford D, Hardy MA, Guarnera GT, Goldstein AL. The regenerative peptide thymosin ?4 accelerates the rate of dermal healing in preclinical animal models and in patients. Ann N Y Acad Sci. 2012 Oct;1270:37-44.

By the way a member who knows The patent holder and is still his patient had chance to verify my experience with Human Relaxin-2. I have managed to source it 2 times and both times it was the same for both vials. I had a specific feeling of relaxation almost sedation as well as a distinct and pronounced desired to carb up or sugar up. A super bad case of the munchies, especially for corn flakes, lots of corn flakes. Any way both my experiences were the same each time I used a vial and every time I took a shot of it… Anyway he told the Dr of my experience and he told him that is consistent with the real deal. And one source for HR2 has been mentioned on this board, so I wont name names, but its out there and you can find it. And if you do get it you might notice something like I did.

Inuic

Originally Posted by inuic
Anyone done any research on topical Glutathione?

Glutathione through glans penis

This might be something if it can impact collagen linking and unlinking…

Inuic

I put it in my pumping water for a good 2 months. No effect on size at that dosage. I do think it helped my skin heal a little faster (red dots and the like), but it was hard to evaluate - a small difference at best.

Got a good chuckle out of the info page about the still-on extender:
“Extended use of the STILL-ON System equipped with the Intacto device will allow you to add to your current length by pulling out the hidden penis from inside of the body.”
ah, the mythical hidden penis..

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